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N = 4 Spry1 +/− and 3 Spry null mice, Means ± SE. The overall volume of the tail vein in both groups of mice is constant. This is further supported by the fact that the non-ischemic leg has a slightly higher blood vessel volume on days 1 and 8 post surgery. On the ischemic side the Spry null mice had a slightly higher blood vessel volume one day post surgery compared to Spry1 +/− control mice, suggesting that these mice are either not as susceptible to this kind of injury or that these mice have higher vascularized hindlimbs compared to their wild type control mice. A) Quantification of blood vessel volumes in the ischemic and nonischemic leg, and tail. VolCal was used to analyze the recovery from hindlimb ischemia in Spry1 null and their respective Spry1 +/− control mice. In summary, we provide a novel, fast and interactive quantification of blood vessel volumes for single blood vessels or sets of vessel segments with particular focus on collateral formation after an ischemic insult.ģD analysis Magnetic resonance angiogram blood vessel volume analysis mouse hindlimb ischemia.
#Vessel volume calculator software#
In clinical settings this software may enhance image interpretation and the speed of data analysis and thus enhance intervention decisions for example in peripheral vascular disease or aneurysms. Using MRAs of the mouse hindlimb ischemia model, we demonstrate quick and reproducible blood vessel volume calculations with 95 - 98% accuracy. The novel plugin named Volume Calculator (VolCal), accepts any binary (thresholded) image and produces a three-dimensional schematic representation of the vasculature that can be directly manipulated by the investigator. In order to enhance analysis options and to provide an automated and fast analysis method, we developed a software plugin for the ImageJ and Fiji image processing frameworks that enables the quick and reproducible volume quantification of blood vessel segments.
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This fact, when combined with a study that involves multiple scans of multiple subjects, can represent a significant portion of research time.
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Volume of an Torispherical Head (V): The volume is returned in cubic meters and the diameter is also returned in meters. INSTRUCTIONS: Choose units and enter the following: The Volume of an Torispherical Head calculator computes the volume of an torispherical head (dish-head) based on the crown radius (R), knuckle radius (a) and the height (h).